napari_prism.pl.preview_tma_segmentation

napari_prism.pl.preview_tma_segmentation#

napari_prism.pl.preview_tma_segmentation(sdata, image_name, segmentation_channel, color_order, channel_merge_method='max', optional_nuclear_channel=None, reference_coordinate_system='global', auto_contrast=True)#

Displays a visual preview of the input data given to Cellpose for segmentation. This is useful for checking if the provided markers provide a good representation for nuclei, cell membranes, cytoplasms, etc.

Parameters:
  • sdata (SpatialData) – The SpatialData object containing the image.

  • image_name (str) – The name of the image to display.

  • segmentation_channel (str | list[str]) – The channel(s) to use for segmentation.

  • color_order (list[str]) – The order of colors for the RGB image.

  • channel_merge_method (Literal['max', 'mean', 'min', 'sum'] (default: 'max')) – Method to merge channels. Options are “max”, “mean”, “min”, or “sum”.

  • optional_nuclear_channel (Optional[str] (default: None)) – Optional nuclear channel for segmentation.

  • reference_coordinate_system (str (default: 'global')) – Coordinate system to use for rendering.

  • auto_contrast (bool (default: True)) – Whether to apply auto contrast to the image.

Return type:

None